Inside Precision Medicine Marketplace Translational Research NEXTFLEX® Small RNA Sequencing Kit v3 for Illumina® Platforms

PerkinElmer
NEXTFLEX® Small RNA Sequencing Kit v3 for Illumina® Platforms

The NEXTFLEX® small RNA-seq kit v3 uses patented and patent-pending technology to provide a reduced-bias small RNA library preparation solution for Illumina® sequencing platforms with gel-free or low-input options. By using randomized adapters, ligation-associated bias is greatly decreased. A bead-based, dual approach for adapter-dimer reduction eliminates the need for PAGE gel purifications when starting with ≥200 ng of total RNA. Additionally, this kit facilitates low input small RNA library preparation with as little as 1 ng of total RNA. The optional addition of UDI barcodes for small RNA sequencing allows multiplexing of up to 192 samples, all while mitigating the risk of index hopping and spread of signal that can occur on a patterned flow cell. The purity of each index sequence is validated by sequencing for high confidence in the resulting sequencing data quality.

For research use only. Not for use in diagnostic procedures.




PerkinElmer

940 Winter St.
Waltham, MA 02451
800-762-4000
https://www.perkinelmer.com





Features & Specs

  • Completely gel-free protocol with normal input amounts
  • Greater discovery/detection rates reduce sequencing cost
  • Randomized adapters reduce ligation bias, resulting in more accurate data
  • Low input (1 ng) gel-based protocol included
  • Offers greater sequencing depth
  • Use more of your RNA sample – 10.5 µL input volume
  • Barcoded primers are included in the kit allowing multiplexing of up to 48 samples
  • An automation protocol is available for the PerkinElmer® Sciclone® G3 NGS/NGSx and the Zephyr® G3 NGS workstation
  • Functionally validated with Illumina® sequencing platforms

Features & Specs

  • Completely gel-free protocol with normal input amounts
  • Greater discovery/detection rates reduce sequencing cost
  • Randomized adapters reduce ligation bias, resulting in more accurate data
  • Low input (1 ng) gel-based protocol included
  • Offers greater sequencing depth
  • Use more of your RNA sample – 10.5 µL input volume
  • Barcoded primers are included in the kit allowing multiplexing of up to 48 samples
  • An automation protocol is available for the PerkinElmer® Sciclone® G3 NGS/NGSx and the Zephyr® G3 NGS workstation
  • Functionally validated with Illumina® sequencing platforms